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P1–263: Assessment of hippocampal volumetry in a transgenic Alzheimer's disease rat
Author(s) -
Shin Monica,
Parent Maxime,
Wang Seqian,
Fonov Vladimir,
Allard Simon,
Carmo Sonia Do,
Gauthier Serge,
Cuello Claudio,
RosaNeto Pedro
Publication year - 2013
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2013.05.488
Subject(s) - hippocampal formation , subiculum , hippocampus , atrophy , anatomy , fornix , coronal plane , sagittal plane , nuclear medicine , medicine , pathology , dentate gyrus
pial towhite matter surfaces were computed across the entire cerebral cortex using an automated image processing method. An intensity profile was generated along each streamline for b-amyloid, GFAP, and NeuN qIHC volumes, and these profiles were averaged over neuroanatomical regions-ofinterest. Comparisons were performed between Tg and WT groups. Results:Distinct cortical laminar profiles were observed for b-amyloid and astrocytes in the Tg mice. While heavy bands of astrogliosis were typically found in superficial and deep cortex, b-amyloid deposits were most abundant in the mid-cortical layers. We also identified previously unreported differences in the regional, multi-parametric, cortical laminar profiles between Tg andWTmice.Conclusions:Our robust, fully-automated method for extraction of cortical laminar profiles of multiple, co-registered IHC markers across the entire mouse cerebral cortex revealed unique observations regarding the patterns of AD-associated alterations in APP Tg mice. We are currently generating similar laminar profiles of vascular and synaptic density in order to obtain a comprehensive picture of neuronal, glial, and vascular pathological changes. Future studies will interrogate the relationships between these cellular measures and in vivo structural and functional imaging data, which will improve our understanding of the complex pathophysiological process underlying AD.