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P4–262: Neuro‐protective effects of pomegranate juice extracts on 1‐methyl‐4‐phenyl‐1,2,3,6‐tetrahydropyridin (MPTP)–induced neurotoxicity in human neurons
Author(s) -
Essa Mohamed,
Selvaraju Subash,
Braidy Nady,
AlAsmi Abdullah,
AlAdawi Samir,
AlSenawi Hamed,
Vaishnav Ragini,
Guillemin Gilles J
Publication year - 2013
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2013.05.1655
Subject(s) - mptp , tbars , chemistry , glutathione , neurotoxicity , lipid peroxidation , superoxide dismutase , oxidative stress , glutathione peroxidase , antioxidant , catalase , pharmacology , thiobarbituric acid , biochemistry , parkinson's disease , enzyme , toxicity , medicine , biology , organic chemistry , disease
stress resulting in DNA damage, PARP activation, NAD+ depletion and cell death.Methods:We evaluated the effect of a series of pomegranate juice extracts (i.e. helow, malasi, qusum, and hamedh) with antioxidant properties on QUIN induced excitotoxicity on primary cultures of human neurons.Results: We showed that helow and malasi can attenuate QUIN-induced excitotoxicity to a greater extent than qusum and hamedh. Similarly, both helow and malasi were able to attenuate QUIN-induced Ca2+ influx and nNOS activity to a greater extent compared to qusum, and hamedh. All extracts reduced the oxidative effects of increasedNO production thereby reducing the formation of 3-nitrotyrosine and PARP activity, and hence preventing NAD+ depletion and cell death. Conclusions: In addition to the well-known antioxidant properties of these natural phytochemicals, the inhibitory effect of some of these compounds on specific excitotoxic processes such as calcium influx provides additional evidence for the beneficial health effects of pomegranate juice extracts in excitable tissue, particularly within the CNS.