Premium
IC‐P‐040: PET imaging using [18F]florbetaben in Alzheimer's disease mouse models
Author(s) -
Brendel Matthias,
Böning Guido,
Delker Andreas,
Rötzer Christina,
Gildehaus FranzJosef,
Baumann Karlheinz,
Steiner Harald,
Haass Christian,
Herms Jochen,
Bartenstein Peter,
Rominger Axel
Publication year - 2013
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2013.05.041
Subject(s) - genetically modified mouse , partial volume , nuclear medicine , standardized uptake value , medicine , in vivo , pathology , tau pathology , cerebellum , alzheimer's disease , cortex (anatomy) , neuroscience , chemistry , disease , transgene , positron emission tomography , biology , biochemistry , microbiology and biotechnology , gene
7T) using a Fast Imaging with Steady State Precession (FISP; TR/TE: 3000/ 9ms; isotropic 250um voxels; 8 angles). WT (N1⁄43; BM1⁄4588.66109.2 g; Age1⁄422 months) and McGill-R-Thy1-APP (N1⁄45; BM1⁄4739.36140.6g; Age1⁄422 months) male rats hadMRI scans under 2% Isoflurane anaesthesia. Hippocampal 3D manual volumetry was performed using the software Display. Coronal images of commissural fornix and amygdala set the anterior hippocampal landmarks. Segmentation continued posteriorly until the granular layer and CA1 disappeared. Ventricles and the mesencephalic cistern served as the medial and lateral landmarks, respectively. Subiculum set the inferior and posterior hippocampal limits. Adjustments were guided by the correspondent sagittal and lateral views of the brains. Results: Three repetitive measurements were taken from each rat. The analyzed hippocampal volume was 657.1633.5 mm 3 for WT and 564.866.5 mm 3 for TG (14%, P1⁄40.01). The total brain volume was 3185.46117.4 mm 3 for WT and 2846.5696.6 mm 3 for TG (10%; P<0.01). Coefficient of variation (CV) was 8.8% forWTand 2.6% for TG.When corrected for total brain volume, the adjusted hippocampal differences decreased to 4%. Single rater absolute intra-class correlation value of 0.91 and F-value of 32 proved significantly high reproducibility of this manual segmentation method. Conclusions: We propose a reliable and reproducible hippocampal volumetric protocol for rats. The method demonstrated to be useful for assessing group differences between control and transgenic models of AD.