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P2‐096: Quantitative modeling of amyloidogenic processing and its influence by SORLA/SORL1 in Alzheimer's disease
Author(s) -
Schmidt Vanessa,
Baum Katharina,
Lao Angelyn,
Rateitschak Katja,
Teichmann Anke,
Wiesner Burkhard,
Wolf Jana,
Wolkenhauer Olaf,
Willnow Thomas
Publication year - 2012
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2012.05.800
Subject(s) - amyloid precursor protein , amyloid precursor protein secretase , receptor , chemistry , neuroscience , intracellular , microbiology and biotechnology , alzheimer's disease , biochemistry , biology , medicine , disease
(Hippocampus, Frontal, Occipital and Temporal lobes) using western blotting and real time PCR. To confirm whether these changes are due to Alzheimer’s disease pathology or a response to aging, we characterised the changes in sirtuin expression in normal aged wistar rats. Results:We found a significant increase in SIRT2 mRNA and protein expression in the occipital lobe. Our data also shows that SIRT5 is downregulated in the temporal lobe. From our results it seems that SIRT2 is the most abundant sirtuin in the human brain. In the aged female wistar rat brain, we found a significant upregulation in SIRT1, SIRT3, SIRT4, mRNA and protein expression in the frontal lobe, while SIRT2 is upregulated in the occipital lobe consistent with AD. SIRT5 and SIRT6 levels are reduced in the aging hippocampus, and temporal lobe, while SIRT7 was significantly increased in the temporal lobe. Conclusions: This study suggests that aberrant expression levels of sirtuins is present in AD and aging, and may represent metabolic differences between humans and rodents.