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P2‐031: Protein kinase activity profiling on cerebrospinal fluid to find diagnostic biomarkers for Alzheimer's disease
Author(s) -
Rosenberger Andrea F.N.,
Hoozemans Jeroen,
Hilhorst Riet,
Scheltens Philip,
Van der Flier Wiesje,
Vies Saskia M.
Publication year - 2012
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2012.05.734
Subject(s) - kinase , cerebrospinal fluid , biology , signal transduction , protein kinase a , microbiology and biotechnology , extracellular , phosphorylation , biochemistry , neuroscience
Background: Humoral immunity related to neuronand disease-specific cytoskeletal proteins in patients with Alzheimer’s disease (AD) is largely unknown. Methods: Antibodies against three targets [tau, light (NFL) and heavy (NFH) neurofilaments] were measured using ELISA in 80 paired serum and CSF samples from 25 patients with AD fulfilling NINCDSADRDA criteria, 13 patients with other dementias (OD), 25 cognitively normal elderly controls (NC), 17 patients with neuroinflammatory diseases (IC). We estimated intrathecal synthesis of all antibodies according to formula (CSF anti-neurocytoskeletal IgG /serum anti-neurocytoskeletal IgG)/ (CSF total IgG /serum total IgG).Results:TheAD patients had significantly higher intrathecal anti-tau antibodies than in the OD group (p 1⁄4 0.005) or the IC group (P 1⁄4 0.0003) and higher intrathecal anti-NFH antibodies than in the OD group (P 1⁄4 0.046), the NC group (p 1⁄4 0.04) or the IC group (P 1⁄4 0.0007), but not intrathecally synthesized anti-NFL antibodies. All three anti-neurocytoskeletal antibodies in serum or CSF did not differ among four groups with the exceptions for the IC group having them elevated in CSF. Conclusions: These new findings suggest some hints of specific alterations in humoral anti-neurocytoskeletal immunity and selectivity in AD. It may have diagnostic and immunotherapeutic implications.