P1‐296: An updated Aβ systems kinetic model for mouse, monkey and human

icity in Alzheimer’s disease (AD) has been focused on sensory deficit, especially impaired olfactory sensibility. AD might provide pathogenic ally significant causes of olfactory dysfunction, which designated even nonfibrillar Ab peptide deposition within olfactory bulb prior to the deposition within any region of brain. In addition, the olfactory receptor neurons (ORNs) might be affected by Ab peptides and contribute to atypical olfactory sensory dysfunction. Therefore, approaches allowing the simple, direct detection of the AD marker within the ORNs in vitro would be beneficial for the assessment of the diseases status. Methods: We suggest a new technical concept that is capable of evaluating as small as a few femtomolar Ab peptides by using a photo-sensitive field-effect transistor (p-FET) integrated with a selectively optical-transmissible filter. Selenium filter has optically adequate properties for the quantum dots for labeling Ab peptides, which reflects the unwanted range (<600 nm) of the excitation light and is only able to transmit the emitted light (>650 nm). The ORNs including Ab peptide s were lysed and placed on the sensing area of the p-FET.Results:We observed destructively decayed photo-current by the selenium-filtered p-FET compared with the bare p-FET, which confirmed that the selenium filter rarely transmit the excitation beam. Thereafter, we measured the photo-currents generated from the transmitted fluorescent beam through the selenium filter when the quantum dots conjugated with Ab on the filter w ere excited at 550 nm and emitted at 655 nm. Compared with no Ab peptide cases, t he difference of the photo-currents with Ab peptide s is as much apparent as about 10 mA. Conclusions: This study showed that with even slightly small amount of Ab peptides the selenium-filtered p-FET is simply applicable to differentiating the optically tenuous fluorescent beam. In addition, the correlation between the p-FET photo-currents and the various amounts of the quantum dots (Ab peptides) represented a linearity which showed a good conceptual agreement with the theoretically induced equation, consequently assuming that the photo-current would potentially evaluate the small quantity of Ab peptides on the p-FET sensing area.