Premium
P1‐261: Intranasal passive immunization using WGA‐modified oligomer antibodies greatly improves learning and memory in Alzheimer's 5XFAD mice
Author(s) -
Chauhan Neelima,
Xiao Chun,
Davis Francesca,
Lambert Mary,
Viola Kirsten,
Lacor Pascale,
Dravid Vinayak,
Klein William
Publication year - 2012
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2012.05.541
Subject(s) - wheat germ agglutinin , morris water navigation task , nasal administration , endocytic cycle , neuroscience , immunization , antibody , medicine , chemistry , immunology , hippocampal formation , psychology , receptor , lectin , endocytosis
ter maze at both 3 weeks and 15 weeks post-operatively (PO). Immunohistochemical changes in amyloid and tau pathology, as well as microglial activation are currently being quantified in these animals. In addition, another group of 11 month old 3xTgADmice were euthanized at 6 hr post-operative to assess changes in neuroinflammatory cytokines by Luminex and ELISA assays. Results: Behavioral analyses revealed no significant changes in the escape latencies at either 3 or 15 weeks PO but a significant transient effect associated with surgery was found in the probe test at 3 weeks PO, which resolved by 15 weeks PO. The spatial working memory test and the Rotorod motor testing showed no significant changes at 4 months PO. The immunohistochemical and biochemical assay results are underway and will be presented at the meeting. Conclusions: These results are in contrast to our previous study of the same abdominal surgery in the same 3xTgAD mouse model, but conducted under desflurane anesthesia. This suggests that propofol dampened the neuroinflammatory effects of surgery and implies that anesthetic management might modulate the long term outcome after surgery in elderly patients with risk factors for Alzheimer’s disease.