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P4‐041: Quantitative proteomics of Alzheimer's‐like cerebral vasculature in TGF‐ß1 overexpressing mice and its perturbation by pioglitazone
Author(s) -
Badhwar Aman,
Stanimirovic D.B.,
Haqqani Arsalan,
Hamel Edith
Publication year - 2012
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2012.05.1742
Subject(s) - pioglitazone , chemistry , proteome , medicine , endocrinology , receptor , transforming growth factor , biology , biochemistry , type 2 diabetes , diabetes mellitus
Elevation of transforming growth factor-beta 1 (TGF\u3b21), a key extracellular matrix regulator, has been documented in the brain and cerebral vasculature of Alzheimer's disease (AD) patients. Transgenic mice overexpressing TGF\u3b21 in the brain (TGF mice) develop AD-like vascular structural changes, impaired vasomotricity, and compromised neurovascular coupling. We have demonstrated that cerebrovascular dysfunction in both aged and young TGF mice is normalized by the peroxisome proliferator-activated receptor-\u3b3 agonist pioglitazone. Our aims are to (a) characterize the cerebrovascular proteome of TGF mice and its perturbation by pioglitazone using label-free mass spectrometry-based quantitative proteomics, and (b) identify proteins that orchestrate pioglitazone-mediated recovery of cerebrovascular function.Peer reviewed: YesNRC publication: Ye