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P3‐005: Pesticide exposure induces cognitive abnormalities and tau hyperphosphorylation in male rats
Author(s) -
Luo Danju,
Chen Ningning
Publication year - 2012
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2012.05.1223
Subject(s) - neurotoxicity , hyperphosphorylation , synapsin i , synaptophysin , morris water navigation task , pathogenesis , hippocampus , pesticide , biology , medicine , endocrinology , toxicity , phosphorylation , microbiology and biotechnology , immunohistochemistry , biochemistry , agronomy , vesicle , membrane , synaptic vesicle
htau, and JNLP3). Additionally, we examined the total tau and phospho-tau levels in the sera of these mice. Results: All four ELISAs show excellent specificity for mouse tau and human tau, with no reactivity with samples from tau KO mice. The absolute sensitivity of the DA31 capture ELISA was determined to be around 24pg/ml. The assays sensitivity is high enough so that wewere able to detect significant levels of total tau and pSer-202 tau in the sera of htau and JNPL3mice. Both taumousemodels demonstrated an age dependent increase of serum tau. Conclusions: In summary, we have developed a series of sensitive and versatile Low-Tau Sandwich ELISAs that provide useful methods to accurately and objectively quantify the levels of soluble and insoluble total tau and phosphorylated tau in transgenic models. To our knowledge, this is the first method developed that detects measurable levels of total tau and phosphorylated tau in the sera of transgenic mice.