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P2‐013: Curcumin derivatives for amyloid detection using 19F magnetic resonance imaging
Author(s) -
Taguchi Hiroyasu,
Yanagisawa Daijiro,
Morikawa Shigehiro,
Shiino Akihiko,
Inubushi Toshiro,
Hirao Koichi,
Shirai Nobuaki,
Tooyama Ikuo
Publication year - 2011
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2011.05.903
Subject(s) - magnetic resonance imaging , curcumin , nuclear magnetic resonance , chemistry , scanner , tail vein , nuclear medicine , biomedical engineering , imaging agent , materials science , medicine , radiology , optics , physics , biochemistry , in vivo , microbiology and biotechnology , biology
Background: Higuchi and colleagues reported an imaging method using 19F magnetic resonance imaging (MRI) with FSB, which allowed the detection of Aß plaques in AD model mice (Nat Neurosci 8:527, 2005). Although the sensitivity of FSB was very low, this finding indicated that 19FMRI could be a promising tool for the detection of Aß deposition. Recently, we have developed a novel 19F-containing curcumin derivative, FMeC1 as a potential imaging agent (Biomaterials 31:4179, 2010). This study investigated whether FMeC1 is suitable as a 19F-MRI probe to detect Aß deposition in the Tg2576 mouse, a model of AD. Methods: FMeC1 solution was prepared at 10 mg/mL in saline containing 10% Tween 80 immediately before use. Mice were anesthetized with sodium pentobarbital (50mg/kg, i.p.). Subsequently, FMeC1 at a dose of 50 200 mg/kg was administered via the tail vein. For imaging, we used a 7.0 T horizontal-bore MR scanner (Unity Inova; Varian). A home-built circular-type surface coil measuring 1.6 cm in diameter and tuned to both the 1H and 19F frequencies (300 MHz and 282 MHz, respectively) was used to collect the data. The total acquisition time for one data set was 50 min. Results: 19F-MRI displayed remarkable levels of 19F signal in the brain of Tg2576 mice at 4 hours after the injection of FMeC1. While no signals were detected in the brain of wild-type mice. Histological analysis showed penetration of the compound across the bloodbrain barrier and binding to Aß plaques in peripherally injected Tg2576 mice. The distribution of Aß deposits in Tg2576 mice was in accordance with the region of the brain in which the 19F signal was imaged. Conclusions: These findings suggest the usefulness of FMeC1 as a 19F-MRI probe for the detection of amyloid deposition in the brain.