Premium
P1‐099: Serum microglia antibodies potential diagnostic tools
Author(s) -
McRae Amanda,
Baboolal Nelleen,
Davis Gershwin
Publication year - 2011
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2011.05.378
Subject(s) - antibody , medicine , microglia , immunocytochemistry , pathology , paraformaldehyde , immunology , gastroenterology , inflammation
candidate biomarkers.Methods: Some years ago, a sensitive technique, the “Bio-barcode assay”, has been developed (Nam et al., Science 2003, 301(5641):1884-6). In this assay the traditional sandwich ELISA-based technique is combined with amplification techniques known frommolecular biology. We have designed a “Bio-barcode assay” for detection of low abundant proteins in cerebrospinal fluid. In this assay, a sandwich is formed by antibody-coated magnetic microparticles on the one side and both antibodyand oligonucleotide-coated nanoparticles on the other side. After formation of the particle-antigen sandwich, oligonucleotides (the so-called "barcodes") are released and quantified. Results: Our current results demonstrate that it is feasible to couple antibodies against the protein of interest both to nanoparticles and to magnetic microparticles. In addition, oligonucleotides can be coupled to the nanoparticles, together with the antibodies. In a prototype assay, we successfully coupled antiAß antibodies in order to design a Bio-barcode Aß oligomer assay. Conclusions: It is feasible to construct the different elements of the Bio-barcode assay, in order to design an assay for the quantification of Aß oligomers in cerebrospinal fluid.