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P1‐029: A comparison of conventional versus push‐pull microdialysis for the detection of amyloid β in the mouse brain
Author(s) -
Motter Ruth,
Veinbergs Isaac,
Cremers Thomas,
Van der Hart Marieke,
Yu Lisa,
Kooijker Harm,
Flik Gunnar,
Freije Robert,
Brendza Robert,
Tanaka Pearl
Publication year - 2011
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2011.05.309
Subject(s) - microdialysis , extracellular , in vivo , chemistry , interstitial fluid , pathophysiology , extracellular fluid , amyloid beta , pharmacology , biochemistry , pathology , medicine , biology , microbiology and biotechnology , peptide
using a sensitive plate based ELISA system (MSD). Results: Unlike gamma secretase inhibitors, which demonstrate a “potency shift" when the concentration of substrate is very high as in systems overexpressing APP (Burton et al 2008), the potency of GSMs does not demonstrate a systematic shift in overexpressing systems. The pattern of modulation of As peptides was consistent across model systems and specific to each structurally distinct GSM, with some demonstrating a decrease in As42 and an increase in As38 and others demonstrating a decrease in both As42 and 38. Conclusions: The physiological significance of these alterations in cleavage site is not well understood, but appears to be driven by unique interactions of GSMs with gamma secretase.