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P3‐173: Amyloid precursor protein regulates SREBP‐mediated neuronal lipid homeostasis in mice and humans
Author(s) -
Pierrot Nathalie
Publication year - 2011
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2011.05.1614
Subject(s) - sterol regulatory element binding protein , endoplasmic reticulum , golgi apparatus , amyloid precursor protein , microbiology and biotechnology , transcription factor , transgene , genetically modified mouse , biology , cholesterol , chemistry , biochemistry , medicine , gene , alzheimer's disease , disease
Methods: Human APP (hAPP) was expressed in rat cultured of cortical neurons and astrocytes using recombinant adenoviruses. Neo-synthesized cholesterol was quantified after 14 C radiolabelling. The transcription of LDL receptor, HMG-CoA reductase, SREBF1 and SREBF2 genes were monitored by real time PCR. The expression of SREBP in cellular and transgenic mouse models was analyzed by western blotting. Subcellular localisation of SREBP and hAPP was analyzed by immunocytochemistry and their interaction by co-immunoprecipitation assays.