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P3‐012: Association between neuropathology and spatial learning in transgenic hAPPSL × hTAU crossbred mice
Author(s) -
Flunkert Stefanie,
Heinrich Petra,
Molnar Annamaria,
Rabl Roland,
Horvath Adam,
Windisch Manfred,
HutterPaier Birgit
Publication year - 2011
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2011.05.1451
Subject(s) - genetically modified mouse , morris water navigation task , neuropathology , transgene , senile plaques , medicine , biology , endocrinology , pathology , alzheimer's disease , disease , hippocampus , genetics , gene
rodent chew. Plasma and brains were collected from animals of different ages. Brains were sequentially extracted with dietylamine and formic acid to obtain the soluble and insoluble brain fractions, respectively. The levels of different Ab species and other APP processing fragments were analyzed using commercially available ELISA (Invitrogen & Innogenetics) or MSD technologies. Extensive statistical analysis was performed on all data. Results: We found that the levels of both endogenous mouse and transgenic human Ab rapidly increased with age, in both the soluble and insoluble brain pools, preceding the plaque deposition detected by histological analysis. However, the levels of Ab in plasma were stable over all ages studied. The obtained information on Ab levels and variability is used to perform estimations of necessary sample size for upcoming efficacy studies. Conclusions: This extensive biochemical characterization of the Tg2576 mouse model is used to design acute and long-term efficacy studies for pharmacological modulation of amyloid processing and clearance.