Premium
P2‐515: Low energy laser light (632.8 nm) suppresses amyloid‐beta peptide‐induced oxidative and inflammatory responses in astrocytes
Author(s) -
Yang Xiaoguang,
Askarova Sholpan,
Sheng Wenwen,
Chen J.K.,
Sun Albert,
Sun Grace,
Yao Gang,
Lee James
Publication year - 2011
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2011.05.1386
Subject(s) - nadph oxidase , colocalization , oxidative stress , superoxide , chemistry , microbiology and biotechnology , reactive oxygen species , cytosol , superoxide dismutase , biophysics , biochemistry , biology , enzyme
In ADDL preparations, smaller and sparsely distributed spherical structures were observed. When mixed with curcumin, both globulomer and ADDL showed increased fluorescence compared with corresponding nonaggregated Aß samples. The QCM analysis revealed significant frequency decreases in globulomer-immobilized electrodes compared with control when curcumin was injected into the vessel at a final concentration of 10 mM. In contrast to curcumin, thioflavin T, a well-known indicator for mature amyloid fibril structures, showed a significant increase in fluorescence in the presence of ADDL, but not with globulomer. QCM analysis with thioflavin T injected at a final concentration of 30 mM indicated a significant frequency decrease in the fibril-immobilized electrode, but no difference between control and the globulomer-immobilized electrode. Conclusions: We found that curcumin interacts strongly with oligomers such as globulomer and ADDL, although thioflavin T interacts only with ADDL, but not with globulomer. This association may contribute to the attenuation of the oligomerinduced neurotoxicity. Curcumin could therefore provide a novel design concept for therapeutic drugs targeting Aß oligomers.