Premium
O4‐08‐08: Amyloid‐beta Oligomers are Excluded From the Post‐synapses in the Hippocampus of Cognitively Intact Individuals with Severe Alzheimer's Disease Nuropathology
Author(s) -
Bjorklund Nicole L.,
Reese Lindsay C.,
Woltjier Randall,
Taglialatela Giulio
Publication year - 2010
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2010.08.061
Subject(s) - hippocampus , synapse , neuropathology , neuroscience , hippocampal formation , postsynaptic potential , alzheimer's disease , synaptic plasticity , cognitive decline , amyloid beta , dementia , psychology , disease , biology , medicine , receptor
genotype, 34/34 or 33/33, differed between pairs. Adjacent sections from each pair were stained using two LCPs/LCOs: pentamer formyl thiophene acetic acid (pFTAA) and polythiophene acetic acid (PTAA). Fluorescence spectra from tissue sections were recorded with an LSM 510 META confocal laser scanning microscope, and spectral processing was achieved with an LSM Image browser. Results: Using PTAA, we observed that AD patients with 34/34 exhibited a different conformational spectrum for core and cerebrovascular amyloid whereas their respective matched 33/33 pair exhibited indistinguishable conformational spectra between the two amyloid structures. pFTAA, sensitive for different conformations for Ab and NFTs, revealed NFT densities in 34/34 AD patients that were apparently greater than those in 33/33 AD patients. Conclusions: The observation that PTAA core amyloid and cerebrovascular amyloid spectra distinguish the amyloid deposits of APOE 34/34 from APOE 33/33 AD patients supports the hypothesis that APOE genotype modulates amyloid structure. pFTAA holds promise as an especially sensitive reagent for visualizing NFTs. LCOs/LCPs show great potential as research tools for the study of proteinopathies, including the pathogenesis of sporadic AD and possibly the influence of APOE genotype.