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P1‐266: Increasing plasma membrane cholesterol impacts on APP‐Bace1 proximity and lipid‐rafts: a study by TIRF‐FRET‐FLIM and fluorescence correlation spectroscopy (FCS)
Author(s) -
Marquer Catherine,
Devauges Viviane,
Cossec Jack-Christophe,
Liot Géraldine,
Lécart Sandrine,
Humbert Sandrine,
Saudou Frédéric,
Duyckaerts Charles,
Lévêque-Fort Sandrine,
Potier Marie-Claude
Publication year - 2010
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2010.05.818
Subject(s) - total internal reflection fluorescence microscope , förster resonance energy transfer , lipid raft , chemistry , fluorescence correlation spectroscopy , biophysics , mcherry , membrane , amyloid precursor protein secretase , cholesterol , amyloid precursor protein , green fluorescent protein , lipid bilayer , microbiology and biotechnology , fluorescence , biochemistry , alzheimer's disease , biology , medicine , quantum mechanics , molecule , gene , physics , disease , organic chemistry
the ipsilateral side. Conclusions: We provide evidence that synaptic activity can reduce Ab levels and protect synapses of transgenic neurons at 12 but not at 19DIV. The differences observed between 12 and 19DIV reinforce our hypothesis that under normal conditions of synaptic activity intracellular and secreted Ab are efficiently cleared, whereas with aging and progressive intraneuronal Ab accumulation, the cellular machinery involved in Ab clearance becomes impaired. Understanding how synaptic activity modulates Ab homeostasis and synapses as a function of time/age could be critical in better understanding the pathogenesis of AD.