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P1‐172: Overexpression of wild type TDP‐43 in motoneuron of non‐human primate
Author(s) -
Yokota Takanori,
Uchida Azusa,
Sasaguri Hiroki,
Kimura Nobuyuki,
Uchihara Toshiki,
Mizusawa Hidehiro
Publication year - 2010
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2010.05.722
Subject(s) - amyotrophic lateral sclerosis , spinal cord , cytoplasm , biology , wild type , primate , anatomy , pathology , neuroscience , microbiology and biotechnology , medicine , gene , genetics , mutant , disease
blot. Results: N2a mouse neuroblastoma cells transfected with human PrP are permissive to infection with CJD PrP, which can be serially passaged. Critical factors to allow successful infection are: a CJD inoculum with a high level of PrP, matching of the codon 129 polymorphism between the inoculum/cell line and the presence of metal ions. Whether the cell passaged CJD PrP is infectious in human PrP expressing Tg mice is under study. Conclusions: We report a novel tissue culture model of sporadic CJD which can be utilized to study the pathogenesis of human prionoses and develop novel therapeutics.