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P1‐116: Mitochondrial deficits lead to Alzheimer‐like changes in calcium homeostasis
Author(s) -
Gibson Gary E.,
Chen Huan-Lian,
Xu Hui,
Denton Travis,
Shi Qingli
Publication year - 2010
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2010.05.665
Subject(s) - calcium , endocrinology , medicine , calcium metabolism , endoplasmic reticulum , depolarization , biology , mitochondrion , bradykinin , cytosol , fura 2 , microbiology and biotechnology , biochemistry , enzyme , receptor
Background: To determine the effect of TEN on the Mash1 expression in neural stem cells from mouse hippocampui and directional differentiation of its NSCs in vitro. Methods: Mouse neural stem cells were generated from the hippocampi of rats within 24 hours after birth and cultured in EGF and bFGF(20 ng/ml each) in 50-mm uncoated culture flask. The third passage neural stem cells were cultured with TEN medium (5mg/L,20mg/L,100mg/ L). Immunocytochemical technique was used to detect NSE-positive neurons,ChAT-positive neurons and GFAP-positive neurons induced by TEN, also was determined in RT-PCR technique the expression of Mash1 and noggin in NSCs affected by TEN. The statistical significance between group comparisons for behavioral data was determined by Chi-square test and one-way ANOVA. P values <0.05 were considered to be statistically significant. Results: The immunocytochemical and RT-PCR results revealed that ChATpositive neurons and NSEpositive neurons were detected with statistical significance(p<0.05) and GFAP-positive neurons with statistical significance(p < 0.01) were detected induced by TEN. The effect of 20mgl/L group was found stronger than other groups. The expression of Mash1 was increased in 20mgl/L groups than vehicle group(p< 0.05) and the expression of noggin with statistical significance were detected between TEN groups and other groups (p < 0.01). Conclusions: This data suggests that TEN can induce NSCs differentiate into neurons and cholinergic neurons from NSCs in vitro; The expression of Mash1 and noggin can be increased in NSCs by TEN.