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P1‐112: Neuronal activity and APP metabolism in the brain of X11/X11L doubly‐deficient mice
Author(s) -
Saito Yuhki,
Inoue Tsuyoshi,
Suzuki Toshiharu
Publication year - 2010
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2010.05.661
Subject(s) - neuroscience , microbiology and biotechnology , chemistry , biology
fibroblast lines were generated from skin-punch biopsy samples using standard techniques. To generate iPS cells, fibroblasts were infected with OCT4, SOX2, KLF4 and MYC retroviruses. iPS clones were picked on the basis of morphology for expansion and further characterisation. Results: Patient-specific iPS cells have been generated and are currently undergoing validation. Successful reprogramming was judged by morphology and expression of pluripotency -associated markers. Ongoing work aims to apply this technique to a larger cohort of patient samples. We are also differentiating ES and iPS cells into neurons to examine tau isoform expression. Conclusions: ES and iPS cell-derived neurons provide a novel cell model for the study of tau splicing and the affects of coding and splice-site tau mutations on neuronal viability.