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O4‐05‐06: A versatile microglia‐neuron co‐culture system for the identification of anti‐inflammatory neuroprotectants: Application to screening of natural compounds
Author(s) -
Muench Gerald,
Rahmadi Anton,
Maczurek Annette,
Steele Megan L.
Publication year - 2010
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2010.05.493
Subject(s) - microglia , neuroprotection , microbiology and biotechnology , neurite , neuroinflammation , chemistry , reactive oxygen species , signal transduction , programmed cell death , viability assay , tumor necrosis factor alpha , biology , biochemistry , cell , inflammation , apoptosis , pharmacology , immunology , in vitro
In Alzheimer ́s disease, activated microglia secrete cytokines, including Il-1, Il-6 and TNF as well as reactive oxygen and nitrogen species (ROS/RNS). These factors contribute to alterations in neuronal glucose uptake, inhibition of mitochondrial enzymes, impaired axonal transport, and synaptic signaling. In addition, ROS act as signaling molecules in pro-inflammatory redox-active signal transduction.