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P3‐199: Relationship between CSF biomarkers and language fluency in asymptomatic middle‐aged adults at increased risk for Alzheimer's disease: The ESPRIT study
Author(s) -
Blazel Hanna M.,
Barnet Jodi,
Gleason Carey E.,
Dowling N. Maritza,
Hermann Bruce P.,
Johnson Sterling C.,
Zetterberg Henrik,
Blennow Kaj,
Marsh Alyce,
Puglielli Luigi,
Atwood Craig,
Sager Mark A.,
Asthana Sanjay,
Carlsson Cynthia M.
Publication year - 2010
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2010.05.1697
Subject(s) - verbal fluency test , asymptomatic , fluency , biomarker , disease , medicine , neuropsychology , psychology , oncology , clinical psychology , cognition , audiology , psychiatry , biochemistry , chemistry , mathematics education
were obtained from 89 cognitive healthy control subjects, age 30 to 85. Platelets were separated via differential centrifugation, fixed and analyzed with flow cytometry and immunofluorescence with antibodies against tau (Tau-5) and phosphorylated tau (PHF-1). Platelet proteins were immunobloted with the same antibodies, while platelets tau was immunoprecipitated with Tau-5. In a second stage, blood samples were obtained from 30 AD patients and 15 paired age controls and subjected to the same analysis and to platelets APP inmunoblots. Results: Immunofluorescence, blots and immunoprecipitation experiments were positive for the presence of both tau and phosphorylated tau in platelets. Immunoblots showed the presence of high molecular weight fractions that appear to correspond to aggregated forms of tau. The relative amount of tau and phospho-tau were relatively stable at different ages, but the ratio of aggregated species to normal tau was significantly higher in AD patients than controls. A correlation was evidenced between tau species and cognitive impairment. Conclusions: The present is the first description of tau in platelets. The assessment of tau species in platelets provides a tool for establishment of a novel biological marker for AD.