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P2‐289: Role of M1 receptors in Alzheimer's disease
Author(s) -
Medeiros Rodrigo,
Caccamo Antonella,
Baglietto-Vargas David,
Kitazawa Masashi,
LaFerla Frank M.
Publication year - 2010
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2010.05.1339
Subject(s) - muscarinic acetylcholine receptor , transgene , neuroscience , muscarinic acetylcholine receptor m1 , dementia , receptor , alzheimer's disease , psychology , disease , medicine , endocrinology , biology , gene , genetics
pharmacotherapy may complement current therapies. Hence, we aim to investigate the distribution of CB1 in the cortex of AD and controls and correlates with clinical features. Methods: Postmortem brain tissues samples of subjects were obtained from established longitudinal studies with prospectively obtained clinical data. Blocks of thawed tissue from brain areas (BA 9, BA 17, hippocampus, anterior cingulate and caudate) were dissected free of white matter, washed and processed for experiments. Immunoblotting was used to measure protein expression. CB1 receptor function was characterized by means of the [3H]SR141716A saturation binding assay. Results: There are no significant CB1 density changes between AD (n1⁄4 15) and controls (n1⁄4 14). The distribution of CB1 density was: BA9 < Caudate < Hippocampus