Premium
P4‐190: Direct evidences that macroautophagy basal activity is low in cultured neurons
Author(s) -
Magnaudeix Amandine,
Martin Ludovic,
Crochetet Marion,
Lévêque Philippe,
Yardin Catherine,
Terro Faraj
Publication year - 2009
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2009.04.757
Subject(s) - autophagy , microbiology and biotechnology , neurodegeneration , neuroprotection , biology , neuron , cathepsin d , programmed cell death , bafilomycin , astrocyte , proteasome , western blot , neuroscience , medicine , apoptosis , biochemistry , central nervous system , disease , enzyme , gene
neuroblastoma cell line, SH-SY5Y cells, the neurite out growth was examined. Results: Using the combining promoter array, we screened an alpha-synuclein targeted promoter of cdc42 gene. As a member of the Rho family GTPase, Cdc42 controls cell migration and morphogenesis. Overexpression and mutant forms of alpha-synuclein down-regulate the expression of cdc42 and affect cytoskeleton architecture for neurite outgrowth. Conclusions: This study suggests a possible function of alphasynuclein as a transcriptional regulator by direct binding to a promoter of target gene. This novel functional mechanism of alpha-synuclein provides us a new possible clue to develop a new therapeutic strategy for the prevention of neurodegeneration through the regulation of downstream pathway of cdc42.