Premium
P4‐210: G protein coupling of galanin, muscarinic and cannabinoid receptors in areas related to the control of memory and cognitive functions of Alzheimer patients
Author(s) -
Manuel Ivan,
BarredaGomez Gabriel,
Giralt Maria T.,
Ferrer Isidro,
RodriguezPuertas Rafael
Publication year - 2009
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2009.04.676
Subject(s) - galanin , basal forebrain , medicine , endocrinology , muscarinic acetylcholine receptor , dentate gyrus , galanin receptor , neuroscience , hippocampus , population , cannabinoid , cholinergic , biology , chemistry , receptor , neuropeptide , environmental health
was to determine the effects of beta amyloid 1-42 treatment on VDR expression in primary cortical neuron cultures. Methods: Cerebral cortex dissected from brains of Sprague Dawley rat embryos on the embryonic day 16 and cultured. The groups including 48 hours of 6 uM beta amyloid 1-42 treated group and control groups, were established. mRNA isolation and cDNA synthesis performed. The levels of VDR expressions were determined by RTqPCR. Localization of VDR was identified by immunofluorescent immunocytochemistry. Results: Expression of VDR in beta amyloid 1-42 treated group was found decreased when compared with control group. The immunolocalization of VDR was observed in both nucleus and cytoplasm of the primary cultured neurons of cerebral cortex. Conclusions: Relative attenuation in the levels of VDR expression by beta amyloid treatment in primary cortical neurons, might indicate potential role of beta amyloid to prevent probable neuroprotective effects of vitamin D in brain.