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P1‐121: Generation and characterization of neuron‐specific mitochondrial targeted DsRed2 transgenic mouse model to image synapse modification in Alzheimer's disease
Author(s) -
Wang Yi,
Martin Lee J.
Publication year - 2008
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2008.05.708
Subject(s) - green fluorescent protein , transgene , biology , transfection , genetically modified mouse , microbiology and biotechnology , reporter gene , gene , genetics , gene expression
activated. The objective of this study was to determine whether the microgliosis is exaggerated in Tg2576 mice deficient in GRK5, in which fibrillar A is present. Methods: Both quantitative and qualitative immunochemistry methods were used to evaluate the microgliosis and astrogliosis in these animals. Results: Microgliosis was characterized by immunofluorescent staining with antibodies against CD45, CD11b, OX42 and interleukin 1 . Quantitative imaging analysis revealed about two-fold increase of CD45 -microglial cells in both hippocampus and cortex of APPsw / GRK5 /mice than that in APPsw /GRK5 / mice. In addition, significant increase of glial fibrillary acidic protein (GFAP)-positive imunoreactivity was also found in the hippocampus and cortex of APPsw / GRK5 /mice as compared to the APPsw /GRK5 / mice. Consistent with previous observations, the activated microglia and astrocytes were located primarily near or surrounding the fibrillar A deposits. Conclusions: Knockdown GRK5 gene by 50% in the Tg2576 mice resulted in approximately doubled microgliosis, along with significantly exaggerated astrogliosis, in both hippocampus and cortex of these animals.