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P4‐237: Nuclear translocation of FE65 regulated by APP in cells with osmotic stress
Author(s) -
Nakaya Tadashi,
Suzuki Toshiharu
Publication year - 2008
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2008.05.2305
Subject(s) - cytoplasm , fluorescence recovery after photobleaching , osmotic shock , nucleus , microbiology and biotechnology , phosphorylation , cell nucleus , nuclear export signal , chemistry , biophysics , biology , biochemistry , membrane , gene
By utilizing this database we performed algorithmic analysis of genes predicted to contain potential CREB binding sites in their promoters. We applied a multilayered bioinformatic approach to reduce the vast number of predicted genes listed in the database to 58 neuronally expressed bioinformatically confirmed putative CREB targets. Verification of these genes as potential CREB neuronal genes was established experimentally using qRTPCR of cDNA isolated from cortical/hippocampal neurons of Forskolin I.P. injected mice, with the aim of mimicking CREB activation. Results: Five genes showed a greater than 2 fold increase in mRNA expression, 22 genes gave a 1.5-2 fold increase and the rest had no significant effect under forskolin stimulation. Conclusions: We have identified potential neuronspecific CREB target genes that will prove valuable to the underling mechanisms that are responsible for activity dependent changes in neuronal properties, which contribute to synaptic plasticity and long-term memory.