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P4‐175: Loss of phospholipid asymmetry and elevated brain apoptotic protein levels in subjects with amnestic mild cognitive impairment and Alzheimer's disease
Author(s) -
Lange Miranda,
Cenini Giovanna,
Memo Maurizio,
Butterfield D. Allan
Publication year - 2008
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2008.05.2242
Subject(s) - phosphatidylserine , lipid peroxidation , oxidative stress , microbiology and biotechnology , apoptosis , western blot , biology , chemistry , biochemistry , phospholipid , membrane , gene
rosines are considered as markers of oxidative stress on proteins. Several studies have shown differences in carbonylation and nitration of individual proteins in brain and body fluids of AD patients. Methods: We have here assessed total levels of carbonyls and nitrotyrosines in cerebrospinal fluid, serum and plasma from 22 AD patients and 18 age-matched controls using commercially available enzyme immunoassay kits. Results: Decreased protein carbonylation was detected in serum of AD patients as compared to age-matched controls. No differences were observed in the levels of CSF or plasma carbonyls between AD patients and controls. However, decreased CSF protein carbonylation was observed in Apolipoprotein E 4 carriers as compared to non-carriers. Levels of nitrotyrosines did not vary between the groups. Conclusions: According to these data, changes in oxidative metabolism related to the pathogenesis of AD cannot be detected as increased CSF, serum, or plasma protein carbonylation or nitration when compared to age-matched controls. More studies are warranted in order to clarify whether decreased levels of serum carbonyls can be linked to the pathogenesis of AD.