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P3‐387: Effects of EF on proliferation and differentiation of neural stem cells in vitro
Author(s) -
Li Lin,
Yao Rui-qin
Publication year - 2008
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2008.05.1957
Subject(s) - neurosphere , neural stem cell , in vitro , nestin , microbiology and biotechnology , chemistry , stem cell , neurotrophic factors , immunocytochemistry , biology , endocrinology , adult stem cell , receptor , endothelial stem cell , biochemistry
Background: Shen-wu capsule (SW) is a new combination drug composed of 6 kinds of traditional Chinese herbs, which was developed by our lab and now it is under phase III clinical trial in 8 hospitals in China for treating AD. Our studies show that SW improved the learning and memory ability, inhibited the production and deposition of beta-amyloid, as well as decreased the inflammatory reactions, the oxidative stress and apoptosis in the brain, increased energy metabolism and neurotrophic factors and their receptors, and enhanced the functions of the cholinergic system. EF is one of the main components of SW. Our previous studies found that EF obviously decreased the beta-amyloid level in APP695 gene-transfected nerve cells in vitro; enhanced learning and memory ability and inhibited beta-amyloid formation in APP transgenic mouse model. The purpose of this study is to investigate the effects of EF on proliferation and differentiation of neural stem cells (NSCs) in vitro. Methods: Fluorescent method was used to detect the BrdU labeling cells; immunocytochemistry was used to detect the nestin, Musashi1, Tuj1, NF-200, GFAP and GalC positive cells. Results: NSCs obtained from neonatal 1 day rats were propagated as neurospheres in serum-free conditioned medium without EGF and bFGF but with EF, and the volume of neurospheres increased gradually from 7 to 28 days. In addition, compared with the FBS control, the number of NF-200 positive neurons significantly increased in the EF groups, and the newborn neurons were morphologically more mature and migrated farther away from neurospheres than those in the FBS control. Conclusions: EF effectively promotes proliferation and differentiation of NSCs in vitro, suggesting that EF may have the new property of regulating CNS function by neurogenesis.