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P2‐470: Survival and differentiation of adult skin‐derived neuroprecursors in a rat model of age‐related cognitive impairment
Author(s) -
Valenzuela Michael,
Dean Sophia,
Mak Blossom,
Bailey Glynis,
Westbrook Fred,
Sachdev Perminder,
Sidhu Kuldip
Publication year - 2008
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2008.05.1549
Subject(s) - morris water navigation task , cognition , neuroscience , stem cell , population , transplantation , senescence , glial fibrillary acidic protein , medicine , psychology , gerontology , pathology , biology , immunohistochemistry , genetics , environmental health
mer s disease (AD). Up to now no resolutive pharmacological therapy exists and there are increasing arguments regarding the beneficial effect of natural nutrients, able to slow down the progression of the disease. Some have the ability to induce cell proliferation and/or survival. Adult neurogenesis could be a good therapeutic strategy for cognitive aging and neurodegenerative diseases(AD). The aims of this work has been to study the modulatory effect of *LMN diet (cocoa, nuts and other natural extracts) in adult mouse neurogenic brain areas. Methods: 129SV male mice, were feed during 40 days with a standard Harlan 2014 control diet and the same diet containing 9,27% of *LMN. Animals received BrdU injections. Brains were processed for inmunohistological and immunoblots studies. Results: Histological samples of mice feed with *LMN diet, showed a noticeable increasing number of proliferative cells by BrdU and PCNA staining, in the adult neurogenic areas: subventricular zone (SVZ) and subgranular layer of dentate gyrus (sgDG). Using Dcx and PSA-NCAM, an increasing in the undifferentiating neurons were determined in the granular layer of dentate gyrus and in the rostral migratory stream (RMS). The immature oligodendrocytes (Ng2) and astrocytes (GFAP) were also increasing in these brain areas. All these results were corroborated by Western-Blot analysis. Moreover, the several interneurons subpopulations of the olfactory bulb were changed. Increasing the tyrosine hydroxylase, calretinin and calbindin neuronal subpopulations, and decreasing the parvalbumin interneurons. In the dentate gyrus, the granular layer presented a high cell number when comparing with the control animals. Conclusions: All these findings showed that *LMN diet promotes the neurogenesis in the adult mouse neurogenic niches. The differences between interneurons subpopulations of olfactory bulb suggested that, *LMN diet could modulate differentiation process in the RMS. Therefore, *LMN diet could be a promising nutrient that would contribute to the neural replacement and to re-establish the brain function in order to avoid the cognitive decline, the main hallmark in Alzheimer’s disease. *Patent submitted: Reference ES2281270. Acknowledgements: This work has been financed by the Spanish Ministry of Industry, project INGENIO 2010CENIT ref MET-DEV-FUN (2006-2009).