Premium
P2‐355: Trimer Aβ42 gene vaccine elicits a high level of antibody production when delivered with the GAL4‐UAS system and the gene gun
Author(s) -
Qu Bao-Xi,
Fu Min,
Johnston Stephen A.,
Rosenberg Roger N.
Publication year - 2008
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2008.05.1433
Subject(s) - gene gun , microbiology and biotechnology , antibody , trimer , biology , chemistry , elispot , virology , immunology , immune system , t cell , dna vaccination , dimer , immunization , organic chemistry
micromolar concentrations. Immunization of young adult mice with hIVIG at 1g/kg i.p. or i.v. resulted in the formation of neutralizing cross-species antibodies as expected, but tolerance developed after both ways of administration in 4-6 weeks. Intraperitoneal administration of hIVIG once weekly for 14 weeks resulted in significant accumulation of human IgG immunoreactivity in the hippocampus of APP/PS1 mice and in some cases opsonization of amyloid plaques. Conclusions: The data suggest that hIVIG contains antibodies that are able to bind Abeta and induce amyloid degradation if provided at high enough concentrations. Development of crossspecies immune response and blood-brain barrier do not prevent testing these effects in transgenic amyloid producing mice also in vivo.