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P2–155: Development of sensitive and specific ELISAs for three–repeat and four–repeat tau isoforms
Author(s) -
Luk Connie Y.,
Giovani Gavin,
Lees Andrew J.,
Silva Rohan
Publication year - 2006
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2006.05.993
Subject(s) - gene isoform , polyclonal antibodies , tau protein , monoclonal antibody , antibody , recombinant dna , epitope , microbiology and biotechnology , peptide , chemistry , biomarker , antigen , biology , biochemistry , alzheimer's disease , immunology , medicine , disease , gene
increased levels of 27OHC. Among patients with different CNS diseases, most had an increased level of at least one of the two oxysterols. Results: In CSF from 18 AD and 20 MCI patients the levels of 24and 27OHC were significantly increased and 24OHC was significantly positively correlated with T-tau (r 0.55, P 65 ng/L) and A 42 ( 500 ng/L) were set according with literature. In case of AD, the percentage of patients with increased levels of 24OHC, T-tau, P-tau and decreased A 42 were similar (ranging from 55 to 67%). In case of MCI, 50% of the patients had increased CSF levels of 24OHC, whereas only 18% and 24% of this population had increased levels of T-tau and P-tau. The high fraction of MCI patients with increased CSF 24OHC (50%), compared with the smaller number of the same patients with a significant alteration of the levels of the other markers, is consistent with the possibility that 24OHC is a new biomarker with a high negative predictive value in connection with evaluation of patients with cognitive impairment disease.