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P1–218: The dementia risk score—a practical tool to predict dementia risk in 20 years among middle aged persons
Author(s) -
Ngandu Tiia,
Winblad Bengt,
Soininen Hilkka,
Tuomilehto Jaakko,
Nissinen Aulikki,
Kivipelto Miia
Publication year - 2006
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2006.05.595
Subject(s) - dementia , medicine , framingham risk score , vascular dementia , risk factor , population , physical therapy , gerontology , disease , environmental health
of AD. We reported that inhibition melatonin biosynthesis induced tau hyperphosphorylation and spatial memory retention deficits in rats. Objective(s): To explore the effect of inhibiting melatonin biosynthesis on neurofilament phosphorylation in rats and the potential underlying mechanism by using haloperidol, a specific inhibitor of 5-hydroxyindole-Omethyltransferase. Methods: Lateral ventricular and intraperitoneal injections were used for administration of haloperidol. Western blots and immunohistochemisty for phosphorylation of neurofilament, HPLC for detecting the level of melatonin in serum, immunoprecipitation and Plabeling assay for activity of cyclin-dependent kinase 5 (cdk-5). Results: Injection of haloperidol results in significantly decreased levels of serum melatonin, increased neurofilament hyperphosphorylation and activate cdk-5 in rats. Exogenous supplementation of melatonin partially arrests neurofilament hyperphosphorylation and the activity of cdk-5. Conclusions: Inhibition of melatonin biosynthesis induces Alzheimer-like neurofilament hyperphosphorylation, which may involve activation of cdk-5.