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IC–P–115: CoQ10 delays the brain atrophy in transgenic aged mice: In vivo volume MRI study
Author(s) -
Li Geng,
Jack Clifford R.,
Yang Edward S.
Publication year - 2006
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2006.05.2321
Subject(s) - atrophy , hippocampus , magnetic resonance imaging , in vivo , nuclear medicine , placebo , brain size , medicine , pathology , endocrinology , radiology , biology , alternative medicine , microbiology and biotechnology
We tested hypotheses that supplemental intake of coenzyme Q10 (CoQ10) could delay the brain atrophy in transgenic aged mice by volume MR image in vivo. 133 mice (37 APP/PS1, 24 APP, 36 PS1 and 36 wild type) with age of 496 days and weight of 29.92g were administered CoQ10 (1200mg/kg/day) and the vehicle (vegetable oil) via gavage for 8 weeks. High-Performance Liquid Chromatography was used to test the tissue levels of CoQ10 and magnetic resonance (MR) images were used to obtain the volumes of the hemispheres and hippocampi. MRI was performed on a Philips Achiva 3 T with a transmit/receive wrist coil. Parameters for PDW 3D TSE transverse brain images were TR 2000 ms, TE 26 ms, FOV 10 10 mm, Matrix scan 400 400, NSA 12, slice thickness 0.5 mm without slice gap. Regions of interest (ROIs) were manually drawn on the whole volume of the hippocampus (H), hemisphere (HM), and intracranial volume using image processing software Analyze 6.0. In order to correct for normal size differences between animals, volume results were expressed as a ratio relative to the intracranial volume. 19 of the initial mice in the first experiment died before completion of the study. Tissue CoQ10 levels were significantly higher after the treatment. There were no significant differences in the placebo groups between the baseline and 2 months (Fig.1). The hippocampus and hemisphere atrophy was observed in APP/PS1 mice in the placebo group, but no brain atrophy was found in APP/PS1 mice in the treated groups (Fig. 2). The protective effect of CoQ10 on hemispheric volume and hippocampal volume was related to genotype; greater in APP/PS1 than APP, PS1 and wild type mice. CoQ10 appears to have a neuroprotective effect in aged mice. The protective effect was greater in mice which produce more amyloid plaques.