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P3–194: Occludin expression in Alzheimer disease and vascular dementia
Author(s) -
Romanitan Mihaela O.,
Winblad Bengt,
Volkmann Inga,
Bajenaru Ovidiu Al.,
Bogdanovic Nenad
Publication year - 2006
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2006.05.1462
Subject(s) - occludin , tight junction , adherens junction , microbiology and biotechnology , blood–brain barrier , biology , pathology , cell junction , neuroscience , medicine , cadherin , central nervous system , cell , biochemistry
Cairo (Egypt) in 1895 of Italian parents descending from the common ancestor of the PS1-Met146Leu family. Objective(s): To study tau pathology of PS1-Met146Leu Italian branch. Methods: Paraffin-embedded brain sections belonging to two PS1-Met146Leu Italian patients were immunohistochemically stained with anti-phospho tau antibody AT8, anti-3R (RD3) and anti-4Repeats tau (RD4) antibodies. Immunoblot of sarcosylinsoluble tau was also performed. Results: Immunohistochemical staining of the hippocampus and cortex revealed widespread tau pathology. Abundant neuritic plaques, neurofibrillary tangles and neuropil threads were observed in both areas by antibodies AT8 and RD3 while fewer deposits were stained by antibody RD4. Occasional Pick body-like inclusions were observed in the hippocampus; these structures were more numerous in the cortical area although their number was limited, compared to the other type of tau aggregates. Double staining with AT8 and RD4 showed that, differently from Pick bodies found in sporadic Pick disease, the observed inclusions could contain tau with 4 repeats. Immunoblot of sarcosylinsoluble tau extracted from parietal, frontal and temporal cortex showed the 4 bands of 60, 64, 68 and 72 kDa characteristic of Alzheimer’s disease cases. Although by immunohistochemistry RD3 antibody recognized more tau deposits than RD4, dephosphorylation of sarcosyl-insoluble tau with alkaline phosphatase showed the presence of all 6 tau isoforms in all investigated cortical areas. Conclusions: The Italian branch shows only occasional Pick body-like inclusions. Moreover, the presence of all tau isoforms account for a clear AD pathology.In view of the fact that Pick bodies have been indicated as an important feature in the Australian branch of the same family we can conclude that PSI-Met146Leu mutation can present variable neuropathological features suggesting that during centuries environmental and genetic factors can contribute to modify the phenotype.