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P2–333: High resolution structural magnetic resonance images of the entorhinal cortex predict the presence of verrucae in 3D reconstruction volumes in control brains
Author(s) -
Augustinack Jean C.,
Kouwe Andre,
Postelnicu Gheorghe M.,
Wiggins Graham C.,
Frosch Matthew P.,
Fischl Bruce R.
Publication year - 2006
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2006.05.1173
Subject(s) - cytoarchitecture , entorhinal cortex , magnetic resonance imaging , cortex (anatomy) , ex vivo , image resolution , flip angle , anatomy , nuclear medicine , nuclear magnetic resonance , medicine , in vivo , biology , neuroscience , hippocampus , physics , optics , radiology , microbiology and biotechnology
Despite the neuroimaging tools that can provide data in vivo to support a diagnosis of probable Alzheimer’s disease (AD), a diagnosis of definite AD currently rests on the presence and spatial organization of neuropathological markers within specific cytoarchitectural areas including limbic, temporal, occipital cortices in post-mortem brain tissue. Histopathological examination of brain tissue is done using serial sections; thus, spatial topography of pathologic findings is challenging to reconstruct threedimensionally. We have developed novel methods to image ex vivo control brains using ultra high resolution MRI that provides a powerful tool to evaluate cytoarchitecture of medial temporal lobe areas. Images were collected on a 7T whole body MRI scanner based on a Siemens Sonata platform (Siemens Medical Systems, Erlangen, Germany) using either a surface coil or a solenoid coil (28.5mmx44mm,4 turn). A conventional 3D spoiled gradient echo sequence was used with 100um resolution for the block data and 120um for surface coil. For 100um data, fast-low-angle-shot images were acquired with a fixed TE 7.8ms and TR 20ms, with flip angles of 10, 20 or 30 degrees. Our ex vivo studies illustrated that layer II islands are visible in the entorhinal cortex (EC) and that when these MR volumes are reconstructed, the verrucae are observed on the surface of the EC. In these data, the presence or absence of EC islands in MR images reflects the presence or absence of verrucae in the MR reconstructions (n 5), and thus, EC islands in the MR images predict the presence of verrucae with reconstruction. Furthermore, the perforant pathway was studied in one case and imaged on 4.7Tesla (Siemens) scanner in a solenoid coil for diffusion tensor imaging. The perforant pathway’s trajectory was generated with DTI Studio (John Hopkins University) and were mapped 1) crossing the hippocampal fissure and 2) coursing around the hippocampal fissure. These data are the preliminary step that will lead to novel insights for the MR signal properties of control brains and AD and ultimately could provide the foundation for in vivo MRI techniques to detect AD neuropathology.