Assessing genetic diversity of Mycobacterium tuberculosis by spoligotyping and IS6110-based restriction fragment length polymorphism in North India | Zendy

Jitendra Prasad Mathuria | Zendy; Pragya Sharma | Zendy; Pradyot Prakash | Zendy; Jai Kumar Samaria | Zendy; Vishwa Mohan Katoch | Zendy; Shampa Anupurba | Zendy

Open Access

Assessing genetic diversity of Mycobacterium tuberculosis by spoligotyping and IS6110-based restriction fragment length polymorphism in North India

Author(s) -

Jitendra Prasad Mathuria,

Pragya Sharma,

Pradyot Prakash,

Jai Kumar Samaria,

Vishwa Mohan Katoch,

Shampa Anupurba

Publication year - 2016

Publication title -

international journal of mycobacteriology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.53

H-Index - 20

eISSN - 2212-554X

pISSN - 2212-5531

DOI - 10.1016/j.ijmyco.2016.11.015

Subject(s) - restriction fragment length polymorphism , genetics , mycobacterium tuberculosis , genetic diversity , biology , terminal restriction fragment length polymorphism , polymorphism (computer science) , tuberculosis , evolutionary biology , genotype , medicine , gene , environmental health , population , pathology

Molecular epidemiology methods are very useful for differentiating between strains, assessing their diversity, and measuring the prevalence of the most circulating strain in an area. Various molecular typing methods using different molecular markers have been utilized worldwide, such as restriction fragment length polymorphism (RFLP), spoligotyping, Mycobacterial Interspersed Repetitive Unit - Variable Number of Tandem Repeat (MIRU-VNTR), and Double repetitive element-PCR (DRE-PCR) typing, for simultaneous detection and epidemiologic typing of Mycobacterium tuberculosis. The present study is conducted to assess the genetic diversity of M. tuberculosis by IS6110-RFLP and spoligotyping in patients attending a tertiary care hospital in eastern Uttar Pradesh, North India.

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