Open AccessComparative proteomic analysis of sequential isolates of Mycobacterium tuberculosis sensitive and resistant Beijing type from a patient with pulmonary tuberculosis
Author(s) -
Amit Singh,
Anil Kumar Gupta,
Krishnamoorthy Gopinath,
Divakar Sharma,
Prashant Sharma,
Deepa Bisht,
Pawan Sharma,
Sarman Singh
Publication year - 2016
Publication title -
international journal of mycobacteriology
Language(s) - English
Resource type - Journals
eISSN - 2212-554X
pISSN - 2212-5531
DOI - 10.1016/j.ijmyco.2016.10.028
Subject(s) - mycobacterium tuberculosis , tuberculosis , drug resistance , microbiology and biotechnology , proteome , genotyping , efflux , biology , multiple drug resistance , virology , medicine , genotype , gene , bioinformatics , genetics , pathology
In India, tuberculosis (TB) is a foremost health problem, and the emergence of multidrug-resistant (MDR) and extensively drug resistant (XDR) strains of Mycobacterium tuberculosis (M. tuberculosis) has further complicated the situation. Although various mechanisms have been proposed to elucidate the emergence of resistance, our knowledge remains insufficient. The formation of a very complex network and drugs of proteins are countered by their efflux/modification or target over-expression/modification. The analysis of the over-expressed proteins and their qualitative and phenotypic evaluation before and after the development of drug-resistance may be the most appropriate tool to understand the mechanisms of the mechanism of development of drug-resistance. Most studies are performed on distinct strains. Therefore, the objective of this study was to compare the proteomic information of sequential isolates of M. tuberculosis Beijing type from a single patient who developed MDR-TB during the course of anti-tuberculosis therapy.