Identification of nontuberculous mycobacteria by high-performance liquid chromatography from patients in tehran | Zendy

Ali Mirzapour | Zendy; Ali Nazari Alam | Zendy; Saeed Z. Bostanabadi | Zendy; Abdolrazagh Hashemi Shahraki | Zendy; Soltan Ahmed Ebrahimi | Zendy; Masoud Yousefi | Zendy

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Identification of nontuberculous mycobacteria by high-performance liquid chromatography from patients in tehran

Author(s) -

Ali Mirzapour,

Ali Nazari Alam,

Saeed Z. Bostanabadi,

Abdolrazagh Hashemi Shahraki,

Soltan Ahmed Ebrahimi,

Masoud Yousefi

Publication year - 2016

Publication title -

international journal of mycobacteriology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.53

H-Index - 20

eISSN - 2212-554X

pISSN - 2212-5531

DOI - 10.1016/j.ijmyco.2016.09.003

Subject(s) - nontuberculous mycobacteria , mycobacterium fortuitum , microbiology and biotechnology , mycobacterium , mycolic acid , biology , mycobacterium abscessus , mycobacterium tuberculosis , antimicrobial , tuberculosis , mycobacterium chelonae , high performance liquid chromatography , bacteria , medicine , chemistry , chromatography , pathology , genetics

Background/Objectives: The genus Mycobacterium contains over 140 species comprises pathogenic and nonpathogenic strains. Nontuberculous mycobacteria (NTM) causing clinical disease have become increasingly common and more diverse. Widespread features of NTM infection can make the diagnosis difficult. Precise species-level detection can aid in distinguishing environmental contamination from actual infection and, furthermore, can assist in making a choice of antimicrobial therapy.Materials and methods: Mycolic acids extracted from saponified mycobacterial cells were examined by high performance liquid chromatography (HPLC) in addition to phenotypic tests for the identification of 20 clinical isolates of mycobacteria at Masoud Laboratory during 2014–2015.Results: Mycobacterium abscessus (8 isolates), Mycobacterium tuberculosis (6 isolates), Mycobacterium intracellulare (3 isolates) and Mycobacterium fortuitum (3 isolates) were identified in these isolates. The phenotypic tests also confirmed the identity of the clinical isolates.Conclusion: Our results showed that HPLC is a more reliable, rapid, simple, easy-to-perform, cost-effective, and specific identification method compared with other identification procedures like phenotypic tests

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