The evaluation of an identification algorithm for Mycobacterium species using the 16S rRNA coding gene and rpoB | Zendy

Yuko Kazumi | Zendy; Satoshi Mitarai | Zendy

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The evaluation of an identification algorithm for Mycobacterium species using the 16S rRNA coding gene and rpoB

Author(s) -

Yuko Kazumi,

Satoshi Mitarai

Publication year - 2012

Publication title -

international journal of mycobacteriology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.53

H-Index - 20

eISSN - 2212-554X

pISSN - 2212-5531

DOI - 10.1016/j.ijmyco.2012.01.004

Subject(s) - rpob , 16s ribosomal rna , biology , mycobacterium fortuitum , microbiology and biotechnology , ribosomal rna , mycobacterium , gene , genetics , bacteria

Conventional biochemical tests are the standard for the identification of Mycobacterium species, but molecular identifications are becoming more prevalent. The rpoB gene encodes the β-subunit of RNA polymerase and is utilized for the identification of Mycobacterium species. In the present study, a stepwise Mycobacterium species identification algorithm using the 16S rRNA encoding gene and rpoB analysis was evaluated for its effectiveness.

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