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Distribution of HPV‐16 variants among isolates from Paraguayan women with different grades of cervical lesion
Author(s) -
Mendoza Laura,
Picconi María A.,
Mirazo Santiago,
Mongelós Pamela,
Giménez Graciela,
Basiletti Jorge,
Arbiza Juan
Publication year - 2013
Publication title -
international journal of gynecology and obstetrics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.895
H-Index - 97
eISSN - 1879-3479
pISSN - 0020-7292
DOI - 10.1016/j.ijgo.2013.01.020
Subject(s) - squamous intraepithelial lesion , medicine , cervical cancer , gynecology , lesion , phylogenetic tree , pathology , cancer , cervical intraepithelial neoplasia , genetics , biology , gene
Objective To determine the distribution of HPV‐16 variants among Paraguayan women with different grades of cervical lesions. Methods Sixty‐seven HPV‐16‐positive cervical samples obtained from women attending health centers in Paraguay between March 2007 and April 2009 were examined, including 29 low‐grade squamous intraepithelial lesion (LSIL), 29 high‐grade squamous intraepithelial lesion (HSIL), 4 cervical cancer, and 5 normal cytology samples. The specimens were analyzed by PCR‐directed sequencing of a 364‐bp fragment of the long control region of HPV‐16, and a phylogenetic tree was compiled with MEGA 5.0 software. Results Most HPV‐16 variants belonged to the European branch (82%); these variants were detected among 25 of 29 women with LSIL, 22 of 29 women with HSIL, 3 of 4 women with cervical cancer, and all women with normal cytology. Two isolates yielded new variants of the European branch with nucleotide substitutions at positions A7752C and A7810T. Non‐European variants, such as African type 1 (1.5%) and Asian‐American (16.5%), were detected only among women with cervical lesions (4/29, LSIL; 6/29, HSIL; 1/29, cervical cancer). These variants had at least 6 nucleotide substitutions adjacent to or within transcription factor binding sites. Conclusion All branches of HPV‐16 variants were detected among Paraguayan women with cervical lesions.