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Implication of the RAGE–EN‐RAGE axis in endometriosis
Author(s) -
Sharma Indu,
Dhawan Veena,
Saha Subhash Chand,
Rashmi Bagga,
Dhaliwal Lakhbir Kaur
Publication year - 2010
Publication title -
international journal of gynecology and obstetrics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.895
H-Index - 97
eISSN - 1879-3479
pISSN - 0020-7292
DOI - 10.1016/j.ijgo.2010.03.037
Subject(s) - rage (emotion) , endometriosis , stromal cell , messenger rna , receptor , western blot , glycation , inflammation , medicine , gene expression , pathophysiology , endocrinology , immunology , cancer research , gene , biology , biochemistry , neuroscience
Objective To investigate the involvement of the receptor gene for advanced glycation ( RAGE ), its ligand EN‐RAGE , and COX‐2 in endometriosis. Methods The mRNA and protein expression of the corresponding genes were determined from endometriotic cells from 28 study patients and healthy endometrial stromal cells from 20 controls by semiquantitative RT‐PCR and Western blot analysis, respectively, using β‐actin as an invariant control. Results The expression of COX‐2 , RAGE , and EN‐RAGE was significantly increased, as evidenced by the significantly greater mRNA and protein expression in the cells of the study patients ( P < 0.001). Previous treatment for endometriosis did not lessen mRNA and protein expression ( P < 0.001). Conclusion Our findings strengthen the hypothesis of an underlying inflammation in the pathophysiology of endometriosis and suggest exploring anti‐inflammatory therapies as adjunct treatment.