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Isolation of human mesenchymal stem cells from third‐trimester amniotic fluid
Author(s) -
You Qi,
Cai Liying,
Zheng Jianhua,
Tong Xiaojing,
Zhang Dan,
Zhang Yuanlong
Publication year - 2008
Publication title -
international journal of gynecology and obstetrics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.895
H-Index - 97
eISSN - 1879-3479
pISSN - 0020-7292
DOI - 10.1016/j.ijgo.2008.06.012
Subject(s) - mesenchymal stem cell , cd90 , amniotic fluid , andrology , amniotic stem cells , cd31 , endoglin , stem cell , umbilical cord , flow cytometry , fetus , microbiology and biotechnology , biology , chemistry , medicine , immunology , cd34 , embryonic stem cell , pregnancy , adult stem cell , gene , genetics , immunohistochemistry
Objective To determine whether mesenchymal stem cells (MSCs) can be isolated in third‐trimester amniotic fluid (AF) and their differentiation induced. Method Sufficient numbers of MSCs were isolated from the AF of 15 healthy women undergoing cesarean delivery in the third trimester to be cultured and induced to differentiate into osteocytes. Results Reverse‐transcriptase polymerase chain reaction showed the MSCs to express the pluripotency marker gene OCT4 , and flow cytometry showed these cells to be positive for CD29, CD73, CD90, and CD105 and negative for CD31, CD45, and CD61. The MSCs were also determined to be nontumorigenic. Conclusion Multipotent MSCs can be obtained from AF in the third trimester, which may be less dangerous than the second trimester to women and their fetuses.