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Placental stem cell markers in pre‐eclampsia
Author(s) -
Benian Ali,
Uzun Hafize,
Aydın Seval,
Albayrak Mustafa,
Uludağ Seyfettin,
Madazli Riza
Publication year - 2008
Publication title -
international journal of gynecology and obstetrics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.895
H-Index - 97
eISSN - 1879-3479
pISSN - 0020-7292
DOI - 10.1016/j.ijgo.2007.09.023
Subject(s) - medicine , leukemia inhibitory factor , gestational age , cd34 , placenta , preeclampsia , population , andrology , gestation , pregnancy , obstetrics , endocrinology , gynecology , stem cell , fetus , interleukin 6 , biology , inflammation , environmental health , genetics
Objective To investigate the placental CD34, CD44, and leukemia inhibitory factor (LIF) levels in normotensive and pre‐eclamptic women. Method The study population consisted of 21 women with pre‐eclampsia. Twenty normotensive pregnant women served as controls and were matched to pre‐eclamptic patients by gestational age at delivery. Decidual samples obtained from the central part of the placenta were stored at − 70 °C until analysis. CD44 and LIF were quantified in homogenates by enzyme‐linked immunosorbent assay (ELISA), while CD34 was quantified by flow cytometry. Results The pre‐eclamptic and normotensive groups were well matched. There were no significant differences in age, parity, weight, and gestational age at birth between the groups ( P > 0 .05). The mean placental levels of CD34 (6.55 ± 2.48 vs 3.16 ± 1.23), CD44 (385.24 ± 178.85 vs 157.75 ± 31.73, and LIF (140 ± 51.11 vs 96.25 ± 31.62) were significantly higher in pre‐eclamptic compared with normotensive women, respectively ( P < 0.05). Conclusion Higher levels of CD34, CD44, and LIF were found in the placentas of pre‐eclamptic compared with normotensive women.