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Placental tissue cyclo‐oxygenase 1 and 2 in pre‐eclamptic and normal pregnancy
Author(s) -
Börekçi B.,
Aksoy H.,
Toker A.,
Özkan A.
Publication year - 2006
Publication title -
international journal of gynecology and obstetrics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.895
H-Index - 97
eISSN - 1879-3479
pISSN - 0020-7292
DOI - 10.1016/j.ijgo.2006.06.016
Subject(s) - medicine , eclampsia , malondialdehyde , placenta , oxidative stress , lipid peroxidation , endocrinology , pregnancy , proportional hazards model , prostaglandin , andrology , fetus , biology , genetics
Objective : To investigate the activities of the 2 isoforms of prostaglandin synthetic enzyme cyclo‐oxygenase (COX), COX‐1 and COX‐2, in the placental tissue of women with pre‐eclampsia and healthy pregnant women. The relationship between placental lipid peroxidation and the activities of COX‐1 and COX‐2 was also investigated. Methods : Tissue specimens were obtained from pre‐eclamptic women (20 had severe pre‐eclampsia and 38 had mild pre‐eclampsia) and 27 healthy pregnant women who underwent cesarean section before the onset of labor. Malondialdehyde (MDA) levels and COX‐1 and COX‐2 activities were measured in placental tissue homogenates. Results : Mean activities for COX‐1 and COX‐2 were significantly lower in women with severe pre‐eclampsia than in healthy controls ( P < 0.05 and P < 0.01, respectively). COX‐1 and COX‐2 activities were also lower in women with mild pre‐eclampsia than in healthy controls, but the difference was of borderline significance ( P = 0.049 and P = 0.059, respectively). The mean placental MDA level was significantly higher in pregnant women with severe and mild pre‐eclampsia than in healthy pregnant women ( P < 0.01 for both). The correlation analysis showed significant negative correlations between MDA and COX‐1 ( r = − 0.44, P < 0.001) and MDA and COX‐2 ( r = − 0.45, P < 0.001) in the placental tissue of women with pre‐eclampsia. Conclusion : These results suggest that COX‐1 and COX‐2 activities are decreased in the placental tissue of women with pre‐eclampsia, probably by oxidative stress.