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Interleukin‐7 and suppression of local peritoneal immunity in ovarian carcinoma
Author(s) -
Xie X.,
Ye D.,
Chen H.,
Lu W.,
Cheng B.,
Zhong H.
Publication year - 2004
Publication title -
international journal of gynecology and obstetrics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.895
H-Index - 97
eISSN - 1879-3479
pISSN - 0020-7292
DOI - 10.1016/j.ijgo.2003.08.015
Subject(s) - ovarian carcinoma , peritoneal fluid , immunohistochemistry , ovarian cancer , medicine , carcinoma , epithelial ovarian cancer , gastroenterology , pathology , interleukin , andrology , cancer , cytokine
Objectives : To investigate the epithelial ovarian carcinoma (EOC) secretion of interleukin‐7 (IL‐7). Methods : Levels of IL‐7 were assayed by enzyme‐linked immunoadsorbent assay and IL‐7 mRNA, and protein expression in tissues and cell lines were detected by RT‐PCR and immunohistochemistry. Results: The median serum IL‐7 level in patients with EOC (32 cases; 32.49 pg/ml) was significantly higher than that of patients with benign tumors (16 cases; 7.59 pg/ml) and healthy women (16 cases; 10.64 pg/ml) ( P <0.05). The median peritoneal fluid IL‐7 level in patients with EOC (17.39 pg/ml) was slightly higher than that of patients with benign tumors (14.09 pg/ml), but not significantly so ( P >0.05). There were positive correlations between the serum and peritoneal fluid IL‐7 levels in both ovarian cancer and benign group ( P <0.05, both). Only two EOC specimens expressed IL‐7 mRNA, and no IL‐7 protein positive was found in any specimens. Conclusions : Epithelial ovarian carcinoma cells rarely express IL‐7, and IL‐7 levels are decreased in the ascitic fluid of patients with EOC.