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A preliminary evaluation of glial cell line‐derived neurotrophic factor (GDNF) levels in cerebrospinal fluid across various gestational ages and clinical conditions of the neonate
Author(s) -
Rajkumar Rahul,
Bhaya Bhavana,
Mamilla Divya,
Czech Theresa,
Kisseih Esther,
Saini Arun,
Chouthai Nitin
Publication year - 2018
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/j.ijdevneu.2017.10.001
Subject(s) - glial cell line derived neurotrophic factor , cerebrospinal fluid , gdnf family of ligands , neurotrophic factors , medicine , neuroscience , biology , receptor
Abstract Background This study aims to investigate glial cell derived neurotrophic factor (GDNF) levels in newborns’ umbilical cord blood and cerebrospinal fluid across various perinatal growth parameters and clinical conditions. Methods Cord blood from 20 newborns and 58 residual CSF samples (stored after completion of clinical testing) were collected. GDNF levels were determined using GDNF ELISA kits from R&D Systems in triplicates with appropriate controls to eliminate background. Results Cord blood GDNF levels were significantly higher (p = 0.004) in preterm newborns (n = 6) (115.05 ± 57.17, pg/ml) when compared to term newborns (n = 14) (19.67 ± 10.67, pg/ml). GDNF levels in CSF trended (p = 0.07) higher in term newborns (n = 10) (19.56 ± 9.11, pg/ml) when compared to preterm newborns at term or post term corrected gestational ages (n = 5) (14.49 ± 3.53, pg/ml). Conclusions GDNF levels in preterm newborns were higher in cord blood and lower in CSF as compared to term newborns. It is important to further study circulating and CSF‐GDNF levels in newborns at different gestational ages and clinical conditions.