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SFPQ associates to LSD1 and regulates the migration of newborn pyramidal neurons in the developing cerebral cortex
Author(s) -
Saud K.,
Cánovas J.,
Lopez C.I.,
Berndt F.A.,
López E.,
Maass J.C.,
Barriga A.,
Kukuljan M.
Publication year - 2017
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/j.ijdevneu.2016.12.006
Subject(s) - cerebral cortex , neuroscience , biology , small hairpin rna , progenitor cell , microbiology and biotechnology , progenitor , cortex (anatomy) , gene knockdown , stem cell , apoptosis , genetics
The development of the cerebral cortex requires the coordination of multiple processes ranging from the proliferation of progenitors to the migration and establishment of connectivity of the newborn neurons. Epigenetic regulation carried out by the COREST/LSD1 complex has been identified as a mechanism that regulates the development of pyramidal neurons of the cerebral cortex. We now identify the association of the multifunctional RNA‐binding protein SFPQ to LSD1 during the development of the cerebral cortex. In vivo reduction of SFPQ dosage by in utero electroporation of a shRNA results in impaired radial migration of newborn pyramidal neurons, in a similar way to that observed when COREST or LSD1 expressions are decreased. Diminished SFPQ expression also associates to decreased proliferation of progenitor cells, while it does not affect the acquisition of neuronal fate. These results are compatible with the idea that SFPQ, plays an important role regulating proliferation and migration during the development of the cerebral cortex.