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Maternal administration of magnesium sulfate promotes cell proliferation in hippocampus dentate gyrus in offspring mice after exposing to prenatal stress
Author(s) -
Xiang Xinli,
Zhao Depeng,
Gao Chonglan,
Wang Kai,
Zhou Qian,
Kang Jiuhong,
Duan Tao
Publication year - 2017
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/j.ijdevneu.2016.12.002
Subject(s) - dentate gyrus , medicine , endocrinology , offspring , hippocampal formation , corticosterone , hippocampus , prenatal stress , fetus , saline , amniotic fluid , chemistry , biology , pregnancy , hormone , genetics
Prenatal stress (PS) inhibits cell proliferation in the hippocampal dentate gyrus (DG), which is related to hippocampal anatomy and function abnormality. The aim of the study was to investigate the effects of magnesium sulfate (MgSO 4 ) on PS‐induced cell proliferation suppression in offspring during embryonic stage and postnatal spatial learning. MgSO 4 administration was performed after PS treatment on pregnant mice. Mice were randomly divided into four groups: non‐PS or PS maternal mice injected with MgSO 4 or saline (P + NS, P + MG, C + MG and C + NS group). Corticosterone was collected from amniotic fluid of mother mice on day 17 of embryonic stage (E17). The ability for spatial learning and memory of pups postnatal 3 week was evaluated using water maze assay. Additionally, cell proliferation was detected by assessing the expression of Ki67 using immunohistochemistry in mice fetuses or pups. PS significantly increased corticosterone level in amniotic fluid ( P < 0.05) and impaired the spatial learning and memory (P + NS vs C + NS of latency time and track path length: P < 0.05) of offspring on postnatal day 21. However, MgSO 4 administration could reverse PS‐induced spatial learning and memory disability (P + MG vs P + NS, P < 0.05). Additionally, PS reduced the number of Ki67‐positive cell in hippocampal DG on E17, E19 and postnatal day 21 (P + NS vs C + NS, P < 0.05), which were also abrogated by maternal administration of MgSO 4 (P + MG vs P + NS, P < 0.05). Collectively, prenatal administration of MgSO 4 can reverse PS‐induced reduction of cell proliferation in hippocampal DG during embryonic stage and postnatal spatial learning.