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Effect of prenatal and early life paracetamol exposure on the level of neurotransmitters in rats—Focus on the spinal cord
Author(s) -
BlecharzKlin Kamilla,
JoniecMaciejak Ilona,
Jawna Katarzyna,
Pyrzanowska Justyna,
Piechal Agnieszka,
Wawer Adriana,
WidyTyszkiewicz Ewa
Publication year - 2015
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/j.ijdevneu.2015.09.002
Subject(s) - spinal cord , monoamine neurotransmitter , dopaminergic , medicine , anesthesia , central nervous system , neurotransmitter , dopamine , endocrinology , serotonin , receptor , psychiatry
Abstract The present study has examined the influence of the prenatal and early life administration of paracetamol on the level of neurotransmitters in the spinal cord of rat pups. The effect of the drug was evaluated in 2‐month old Wistar male rats exposed to paracetamol in doses of 5 (P5, n = 9) or 15 mg/kg (P15, n = 9) p.o. during the prenatal period and after birth until the completion of the second month of life. A parallel control group received tap water (Con, n = 9). In this study we have determined the level of monoamines, their metabolites and amino acids in the spinal cord of rats using high performance liquid chromatography (HPLC) in the second month of life. The present experiment demonstrates the action of paracetamol at the molecular level associated with significant modulation of neurotransmission in the spinal cord related to dopaminergic and noradrenergic systems. Simultaneously, paracetamol administration increases the content of an aspartic and glutamic acids in the spinal cord at a critical time during development.